Genome structure and de novo synthesis of poliovirus

• Yogo, Y. and Wimmer, E. Sequence Studies of Poliovirus RNA. II. Polyadenylic Acid at the 3'-Terminus of Poliovirus RNA. Proc. Natl. Acad. Sci. USA 69:l877-l882 (l972).
• Lee, Y. F., Nomoto, A., Detjen, B. M. and Wimmer, E. A Protein Covalently Linked to Poliovirus Genome RNA. Proc. Natl. Acad. Sci. USA 74:59-63 (l977).
• Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., Anderson, C. W. and Wimmer, E. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 29l:547-553 (l98l).
• van der Werf, S. Bradley, J. Wimmer, E., Studier, F. W., and Dunn, J. J. Synthesis of Infectious Poliovirus RNA by Purified T7 RNA Polymerase. Proc. Natl. Acad. Sci. USA, 83:2330-2334 (1986).
Molla, A., Paul, A.V., and Wimmer, E. Cell-free, de novo synthesis of poliovirus. Science 254:1647-1651 (1991).
• Cello, J., Paul, A., and Wimmer, E. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. Science 297:1016-1018 (2002).

Translational control

• Jang, S. K., Kräusslich, H.-G., Nicklin, M. J. H., Duke, G. M., Palmenberg, A. C. and Wimmer, E. Evidence in Vitro for Internal Entry by the Translational Machinery in the 5' Non-Translated Region of Encephalomyocarditis Virus RNA. J. Virol. 62:2636-2643 (1988).
• Jang, S.K., Davies, M.V., Kaufman, R.J. and Wimmer, E. Initiation of Protein Synthesis by Internal Entry of Ribosomes into the 5' Non- translated Region of Encephalomyocarditis Virus RNA In Vivo. J. Virol., 63:1651-1660 (1989).
Lu, H.-H., and Wimmer, E. Poliovirus Chimeras Replicating Under the Translational Control of Genetic Elements of Hepatitis C Virus Reveal Unusual Properties of the Internal Ribosomal Entry Site of Hepatitis C Virus. Proc. Natl. Acad. Sci. (USA) 93:1412-1417 (1996).
• Zhao, W.-D., and Wimmer, E. Genetic Analysis of the Internal Ribosomal Entry Site (IRES) of Hepatitis C Virus in a Poliovirus Chimera: Evidence for a Novel Structure of Domain II. J. Virol. 75:3719-3730 (2001).

RNA replication

• Nomoto, A., Detjen, B., Pozzatti, R. and Wimmer, E. The Genome-Linked Protein of Picornaviruses. II. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature 268:208-2l3 (l977).
• Paul, A.V., v. Boom, J., Filippov, D., and Wimmer, E. Protein-primed RNA Synthesis by Purified Poliovirus RNA Polymerase. Nature 393:280-284 (1998)
• Paul, A.V., Rieder, E., Kim, D.W., van Boom, J.H., and Wimmer E. Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg. J. Virol. 74:10359-10370 (2000)
• Paul, A.V. Possible unifying mechanism of picornavirus genome replication. In "Molecular Biology of Picornaviruses", B.S. Semler and E. Wimmer, eds. ASM Press 2002, pp 227-246.
• Paul, A.V., Yin, J., Mugavero, A., Rieder, E., Liu, Y., and Wimmer, E. A "slide-back" mechanism for the initiation of protein primed RNA synthesis by the RNA polymerase of poliovirus. J Biol Chem. 278:43951-60 (2003)
• Duggal, R., Cuconati, A., Gromeier, M., and Wimmer E. Genetic Recombination of Poliovirus in a Cell-Free System. Proc. Natl. Acad. Sci. (USA) 94: 13786-13791 (1997).

Poliovirus Receptor CD155 and Pathogenesis

• Mendelsohn, C.L., Wimmer, E., and Racaniello, V.R. Cellular Receptor for Poliovirus: Molecular Cloning, Nucleotide Sequence, and Expression of a New Member of the Immunoglobulin Super-family. Cell, 56, 855-865 (1989).
• Bernhardt, G., Harber, J., Zibert, A., deCrombrugghe, M, and Wimmer, E. The Poliovirus Receptor: Identification of Domains and Amino Acid Residues Critical for Virus Binding. Virology, 203:344-356 (1994).
• Harber, J., Bernhardt, G., Lu, H.-H., Sgro, J.-Y., and Wimmer, E. Canyon Rim Residues, Including Antigenic Determinants, Modulate Serotype-Specific Binding of Polioviruses to Mutants of the Poliovirus Receptor, Virology, 214:559-570 (1995).
• He, Y., Valorie D. Bowman, Steffen Mueller, Carol M. Bator, Jordi Bella, Xiaozhong Peng, Timothy S. Baker, Eckard Wimmer, Richard J. Kuhn, and Michael G. Rossmann. Interaction of the Poliovirus Receptor with Poliovirus. Proc. Natl. Acad. Sci. (USA) 97:79-84 (2000).
• Gromeier, M., Alexander, L., and Wimmer, E. IRES Substitution Eliminates Neurovirulence in Intergeneric Poliovirus Recombinants. Proc. Natl. Acad. Sci. (USA); 93:2370-2375 (1996).
• Gromeier, M., and Wimmer, E. Mechanism of Injury-Provoked Poliomyelitis. J. Virol. 72:5056-5060 (1998).
• Gromeier, M., Lachmann, S., Rosenfeld, M. R., Gutin, P. H., and Wimmer, E. Intergeneric Poliovirus Recombinants for the Treatment of Malignant Glioma. Proc. Natl. Acad. Sci. (USA) 97:6803-6808 (2000).
• Mueller, S., Cao, X., Welker, R., and Wimmer, E. Interaction of the poliovirus receptor CD155 with the dynein light chain Tctex-1 and its implication for retrograde axonal transport in poliovirus pathogenesis. J. Biol Chem. 277:7897-7904 (2002).
• Iwasaki, A., Welker, R., Mueller, S., Nomoto, A., and Wimmer, E. Immunofluorescence Analysis of Poliovirus Receptor Expression in Peyer's Patches of Humans, Primates and CD155 Transgenic Mice: Implication for Poliovirus Infection. J. Inf. Diseases 186:585-592 (2002).
• Mueller, S., and Wimmer, E. Recruitment of Nectin-3 to cell-cell junctions through trans-heterophilic interaction with CD155, a vitronectin and poliovirus receptor that localizes to avb3 integrin containing membrane microdomains. J. Biol. Chem., 278:31251-31260 (2003)